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You are here:Home » Antibodies » Antibodies-Cancer Markers » Anti -p21, WAF1 (Cip1, Sdi1, Pic1,Wild-type p53-activated Fragment, Tumor Suppressor Protein) (BSA & Azide Free)

Anti -p21, WAF1 (Cip1, Sdi1, Pic1,Wild-type p53-activated Fragment, Tumor Suppressor
Protein) (BSA & Azide Free)


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Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IP IH FC
Expression of p21WAF1 is induced by wild type, but not mutant, p53 suppressor protein. The p21WAF1 protein binds to cyclin/CDK complexes and inhibits their kinase activity thereby stopping cell cycle progression. Meanwhile, the p21WAF1 protein also binds to PCNA (proliferating cell nuclear antigen) and blocks DNA replication but not the DNA repair process. Evidently, inactivation of p53 disrupts the p53-dependent expression of p21WAF1. p21WAF1is a tumor suppressor involved in the pathogenesis of a variety of malignancies.
Catalog #P1000-55X
ApplicationsSuitable for use in Flow Cytometry, Immunohistochemistry (formalin/paraffin), and Immunoprecipitation. Other applications have not been tested.
Recommended DilutionImmunohistochemistry: 2-4ug/ml for 30 minutes at room temperature.
Immunoprecipitation: 2ug/mg protein lysate. Co-precipitates cdk4.
[Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.]
Optimal dilutions to be determined by the researcher.
Positive ControlLs174T or Raji cells and colon carcinoma.
Cellular LocalizationNuclear
Molecular Weight of Antigen21kD
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
Clone No0.N.489
FormSupplied as a liquid in 10mM PBS, pH 7.4. Also supplied with BSA and sodium azide. See P1000-55.
PurityPurified by Protein G affinity chromatography.
ImmunogenFull length human recombinant p21 protein.
SpecificityRecognizes Human, bovine, Mouse and Rat p21WAF1/Cip1/Sdi1/Pic1 tumor suppressor protein.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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