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You are here:Home » Antibodies » Abs to Protein Kinase Inhibitors » Anti -p27Kip1

Anti -p27Kip1

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP
p27 Kip1 is a member of the Cip/Kip family of cyclin-dependent kinase inhibitors. Like its relatives, p57 Kip2 and p21 Waf1/Cip1, its ability to enforce the G1 restriction point is derived from its inhibitory binding to cdk2/cyclin E and other cdk/cyclin complexes. Expression levels of p27 are upregulated in quiescent cells and in cells treated with cAMP or other negative cell cycle regulators. Downregulation of p27 can be induced by treatment with interleukin 2 or other mitogens. This involves phosphorylation of p27 and its degradation by the ubiquitin-proteasome pathway.
Catalog #P1001-05B
ApplicationsSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000
Immunoprecipitation: 1:100
Optimal dilutions to be determined by the researcher.
Storage and StabilityFor long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
PurityPurified by Protein A affinity chromatography.
ImmunogenSynthetic peptide corresponding to the sequence of human p27 (KLH).
SpecificityRecognizes total levles of human endogenous p27 protein at 27kD. May recognize p57 Kip2. Species Crossreactivity: mouse and rat
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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