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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified B IP

p27 Kip1 is a member of the Cip/Kip family of cyclin-dependent kinase inhibitors. Like its relatives, p57 Kip2 and p21 Waf1/Cip1, its ability to enforce the G1 restriction point is derived from its inhibitory binding to cdk2/cyclin E and other cdk/cyclin complexes. Expression levels of p27 are upregulated in quiescent cells and in cells treated with cAMP or other negative cell cycle regulators. Downregulation of p27 can be induced by treatment with interleukin 2 or other mitogens. This involves phosphorylation of p27 and its degradation by the ubiquitin-proteasome pathway.
Catalog #	P1001-05B
Applications	Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.
Recommended Dilution	Western Blot: 1:1000
	Immunoprecipitation: 1:100
	Optimal dilutions to be determined by the researcher.
Storage and Stability	For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Human
Concentration	As reported
Form	Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
Purity	Purified by Protein A affinity chromatography.
Immunogen	Synthetic peptide corresponding to the sequence of human p27 (KLH).
Specificity	Recognizes total levles of human endogenous p27 protein at 27kD. May recognize p57 Kip2. Species Crossreactivity: mouse and rat
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.