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You are here:Home » Antibodies » Abs to Protein Kinase Inhibitors » Anti -p27kip1, phosphophorylated (Thr187) (Cyclin-dependent Kinase Inhibitor 1B, Cyclin-dependent Kinase Inhibitor p27, CDKN1B, KIP1)

Anti -p27kip1, phosphophorylated (Thr187) (Cyclin-dependent Kinase Inhibitor 1B,
Cyclin-dependent Kinase Inhibitor p27, CDKN1B, KIP1)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IH
Cyclin-dependent kinase inhibitor 1B (CDKN1B), also known as p27 (KIP1), is a cyclin-dependent kinase (Cdk) inhibitor implicated in G1 phase arrest, which negatively regulates G1 phase progression in response to TGF beta and represents a tumor suppressor gene. Human p27 gene is mapped to chromosome 12p12.3 p27 can be both an inhibitor and a substrate of cyclin E-CDK2. p27, abundant in quiescent cells and drops after serum stimulation, plays a role in mediating VSMC hypertrophy. p27 degradation is subject to dual control by the accumulation of both SKP2 and cyclins following mitogenic stimulation. It regulates cell proliferation by binding to and modulating the activity of cyclin-dependent kinases. Reduced p27 activity is fundamental for the development of many human malignancies including breast, prostate, colon and gastric carcinomas.
Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Catalog #P1001-05W
Recommended DilutionWestern Blot:1:500-1:1,000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic phosphopeptide corresponding to human p27Kip1 around the phosphorylation site of threonine 187 (E-Q-TP-P-K).
SpecificityRecognizes human p27kip1 when hosphorylated at threonine 187. Species Cross-Reactivity: mouse, rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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