Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Antibodies » Abs to Protein Kinase Inhibitors » Anti -p27Kip1 (Cyclin-dependent Kinase Inhibitor 1B, Cyclin-dependent Kinase Inhibitor p27, CDKN1B, KIP1)

Anti -p27Kip1 (Cyclin-dependent Kinase Inhibitor 1B, Cyclin-dependent Kinase Inhibitor
p27, CDKN1B, KIP1)

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP IH FC IF
p27Kip1, a candidate tumor suppressor gene, functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF-b induced G1 arrest.
Catalog #P1001-06C
ApplicationsSuitable for use in Western Blot, Immunoprecipitation, Immunofluorescence, Flow Cytometry and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1ug/ml for 2hrs at RT
Immunoprecipitation: 2ug/mg protein lysate
Immunohistochemistry (Formalin fixed paraffin embedded): 1:50 for 30min at RT
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10min followed by cooling at RT for 20min
Optimal dilutions to be determined by the researcher.
Positive ControlColon carcinoma, MAD109, Ls174T cells
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone NoSPM348
HostMouse
SourceMouse
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.05% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant corresponding to mouse p27.
SpecificityRecognizes mouse p27. Species Crossreactivity: human, canine and rat. Does not react with bovine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links