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Anti -p27Kip1 (Cyclin-dependent Kinase Inhibitor 1B, Cyclin-dependent Kinase Inhibitor p27, CDKN1B, KIP1)
Anti -p27Kip1 (Cyclin-dependent Kinase Inhibitor 1B, Cyclin-dependent Kinase Inhibitor
p27, CDKN1B, KIP1)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
B IP IH |
|
| The progression through the cell cycle is regulated by cyclins and their cognate Cdks by promoting cell cycle transitions. This orderly progression can be inhibited by a family of proteins known as CDK inhibitors (CDIs) that bind to cyclin/Cdk complexes and halt cell division. p21 (also designated WAF1/Cip1) is one has been shown to inhibit the activity of each member of the cyclin/Cdk family and over expression of this protein inhibits the proliferation of mammalian cells. The expression of p21 is inducible by a wide range of stress stimuli and its transcription can be enhanced by p53. Another member of the CDIs is p27 (also known as Kip1), which also sees up regulation in response to antimitogenic stimuli. The increased protein expression of p27 results in cellular arrest by binding to cyclin/Cdk complexes, like cyclin D1/Cdk4. An additional CDI has been found to bind Cdk4 and Cdk6, p16 (INK4A), and when such complexes are formed, the progression of the cell cycle is halted. It has become increasingly clear that p16 is a very important tumor suppressor gene whose frequent loss occurs early in many human cancers. p16 is a major target in carcinogenesis that is rivaled in frequency only by p53. | | | Catalog # | P1001-06D | | Applications | Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. | | Recommended Dilution | Western Blot: 1-2ug/ml | | Immunoprecipitation: 3-5ug/extract from 10 cells | | Immunohistochemistry: 1:100 @ RT | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Polyclonal | | Host | Rabbit | | Source | Human | | Concentration | ~0.1mg/ml | | Form | Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | Synthetic peptide corresponding to the C-terminus of human p27Kip1. | | Specificity | Recognizes human p27Kip1. Species Crossreactivity: rat. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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