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You are here:Home » Antibodies » Antibodies-Protein Kinase Inhibitors » Anti -p27Kip1, phosphorylated, (Ser10)

Anti -p27Kip1, phosphorylated, (Ser10)


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Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP IF
Cyclin dependent kinases (Cdk) govern cell cycle progression in eukaryotic cells. Various cyclin dependent kinase inhibitors may suppress the activity of these proteins. There are two families of Cdk inhibitors, the cip/kip (kinase inhibitor protein) and ink (inhibitor of CDK4) families. The cip/kip inhibitors share sequence homology and can inhibit the kinase activities of a variety of Cdks. Cdk inhibitor p27(Kip1) is a member of the kip family and negatively regulates G1 progression by binding to cyclin-Cdk2 complexes. The concentration and subcellular localization of p27 controls its activity. Cellular abundance of p27 is regulated by its degradation via the ubiquitin-proteasome pathway. p27 needs to be transported into the nucleus to exert its inhibitory action. Nuclear import is dependent on the presence of a nuclear localization signal near the C-terminus of the protein, whereas nuclear export into the cytoplasm is dependent on Ser10 phosphorylation. Export into the cytoplasm has been suggested as a mechanism to remove a fraction of p27 from its nuclear targets thereby lowering its effective nuclear concentration below a critical threshold resulting in activation of cyclin-Cdk2 complexes. There have been several reports that indicate p27 (Kip1) mediates multiple cellular functions such as cell proliferation, cell differentiation, and apoptosis. The pivotal role of p27 in these cellular functions makes it important in many disease processes, including neoplasia, and its reduced expression has been shown to correlate with poor prognosis in cancer patients.
Catalog #P1001-09
ApplicationsSuitable for use in Western Blot, Immunopreciptiation and Immunofluorescence. Other applications have not been tested.
Recommended DilutionWestern Blot: 1-3ug/ml
Immunoprecipitation: 5-10ug/IP reaction
Immunofluorescence: 5-10ug/ml
Optimal dilutions to be determined by the researcher
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
FormSupplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide encompassing the phosphorylated Ser10 residue of p27/Kip1.
SpecificityRecognizes p27 when it is phosphorylated at the Ser10 residue. Lysates of HEK 293T cells transfected with an expression plasmid encoding a p27 mutant where Ser10 was substituted with an alanine residue were used to confirm the antibody’s specificity. Reactivity was confirmed with HEK293 cells expressing wild-type p27/KIP that is normally phosphorylated. NIH3T3 and MCF-7 cell lysates can also be used as positive controls.
Species CrossreactivityHuman, rat and mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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