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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E B

The cyclin-dependent kinase inhibitor p27KIP1 is a potent inhibitor of both Cyclin E-Cdk2 and Cyclin A-Cdk2, two cdk complexes involved in the regulation of G1/S transition. p27 is phosphorylated on threonine 187 by cyclin E/Cdk2, and overexpression of this complex in mammalian cells induces degradation of transfected p27. The Cdk-mediated phosphorylation of p27 on threonine 187 is required for p27 ubiquitination and consequent degradation. The phosphorylation on threonine 187 and the ubiquitination of cellular p27, as well as p27 ubiquitinating activity, were found to be regulated collectively during the cell cycle, being present in proliferating cells but not in G0- and G1- arrested cells.
Catalog #	P1001-10A
Applications	Suitable for use in ELISA and Western Blot. Other applications have not been tested.
Recommended Dilution	ELISA: 0.1-1ug/ml
	Western Blot: 1-3ug/ml
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1,k
Clone No	6A153
Host	Mouse
Source	Human
Concentration	0.5mg/ml
Form	Supplied as a liquid in PBS, pH 7.4, containing 0.1% sodium azide
Purity	Purified by Protein A chromatography.
Immunogen	Synthetic peptide encompassing the phosphorylated Thr187 residue of human p27
Specificity	Specific for p27 when phosphorylated at Thr187. On Western blots, it identifies the target band at ~27kD. It does not react with unphosphorylated p27. Specificity has been confirmed with Thr187-mutant p27 recombinant protein. Reactivity has been confirmed with p27-transfected 293 cells. Based on sequence homology, this antibody may also react with mouse, rat and hamster.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.