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Anti -p27Kip1 (Cyclin-dependent Kinase Inhibitor 1B, Cyclin-dependent Kinase Inhibitor p27, CDKN1B, KIP1)
Anti -p27Kip1 (Cyclin-dependent Kinase Inhibitor 1B, Cyclin-dependent Kinase Inhibitor
p27, CDKN1B, KIP1)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Goat |
Affinity Purified |
B |
|
| p27Kip1 gene encodes an inducible inhibitor of cyclin-dependent kinase activity. It associates with cyclin E-Cdk2 complexes in vivo and in vitro, prevents their activation, and inhibits previously activated complexes, and its overexpression obstructs cell entry into S phase. It potently inhibits Rb phosphorylation by cyclin E-Cdk2, cyclin A-Cdk2, and cyclin D2-Cdk4. It is highly conserved and broadly expressed in human tissues, with highest levels in skeletal muscle, lowest in liver and kidney and its mRNA levels are similar in proliferating and quiescent cells. It has a region of sequence similarity to p21Cip1/WAF1, the Cdk inhibitor whose transcription is stimulated by p53. The protein levels are high during G0 and G1 phases of the cell cycle, resulting in cyclin/CDK inhibition. The pathological role of the protein has been observed in multiple endocrine neoplasia type 4. | | | Catalog # | P1001-11A | | Applications | Suitable for use in Western Blot. Other applications not tested. | | Recommended Dilution | Western Blot: 1-3ug/ml | | Optimal dilutions to be determined by the researcher. | | Positive Control | Human Lysate | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Goat | | Source | Human | | Concentration | ~0.5mg/ml | | Form | Supplied as a liquid in Tris saline, 0.02% sodium azide, pH7.3, 0.5% BSA. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | Synthetic peptide corresponding to C-EQTPKKPGLRRRQT, from the C-terminus of the protein sequence according to NP_004055.1 | | Specificity | Recognizes human CDKN1B. Species sequence homology: canine, rat, mouse, bovine. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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