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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified B

The cyclin-dependent kinase (cdk) inhibitor p27 Kip1 is a tumor suppressor protein that acts in the nucleus to enforce cell cycle checkpoints. p27 Kip1 binds to and inhibits many cyclin/cdk complexes, including cyclin D/cdk4, cyclin E/cdk2, and cyclin A/cdk2. This inhibition can subsequently block progression through different phases of the cell cycle. p27 Kip1 is phosphorylated at T198 in cells treated with the AMP-dependent protein kinase (AMPK) activator AICAR (5-amino-4-imidazolecarboxamide riboside) or glucose withdrawal.
Applications:
Western Blot: 0.5ug/ml.
Storage and Stability:
Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile PBS with 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalog #	P1001-11D
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Human
Concentration	As reported
Form	Supplied as a lyophilized powder from a 0.2um filtered solution in PBS with 5% trehalose. Reconstitute with sterile PBS, 40-50% glycerol. If 0.1ml is used, the concentration will be 1mg/ml.
Purity	Purified by immunoaffinity chromatography.
Immunogen	Phospho-peptide containing human p27 Kip1 (T198) site
Specificity	Recognizes human phospho-p27 Kip1 (T198).
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.