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You are here:Home » Antibodies » Antibodies-Protein Kinase Inhibitors » Anti -p27Kip1 (Cyclin-dependent Kinase Inhibitor 1B, Cyclin-dependent Kinase Inhibitor p27, CDKN1B, KIP1)

Anti -p27Kip1 (Cyclin-dependent Kinase Inhibitor 1B, Cyclin-dependent Kinase Inhibitor
p27, CDKN1B, KIP1)


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Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IH
p27Kip1 gene encodes an inducible inhibitor of cyclin-dependent kinase activity. It associates with cyclin E-Cdk2 complexes in vivo and in vitro, prevents their activation, and inhibits previously activated complexes, and its overexpression obstructs cell entry into S phase. It potently inhibits Rb phosphorylation by cyclin E-Cdk2, cyclin A-Cdk2, and cyclin D2-Cdk4. It is highly conserved and broadly expressed in human tissues, with highest levels in skeletal muscle, lowest in liver and kidney and its mRNA levels are similar in proliferating and quiescent cells. It has a region of sequence similarity to p21Cip1/WAF1, the Cdk inhibitor whose transcription is stimulated by p53. The protein levels are high during G0 and G1 phases of the cell cycle, resulting in cyclin/CDK inhibition. The pathological role of the protein has been observed in multiple endocrine neoplasia type 4.
Catalog #P1001-11E
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:500-1:1000
Immunohistochemistry (paraffin): 1:50-1:100
Optimal dilutions to be determined by the researcher.
Positive ControlA2780 and COLO205 cells
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
ConcentrationAs reported
FormSupplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic non-phosphopeptide corresponding to human p27Kip1 around the phosphorylation site of serine 10 (N-G-SP-P-S).
SpecificityRecognizes human p27Kip1. Species Crossreactivity: mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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