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You are here:Home » Antibodies » Abs to Neutrophils » Anti -p47 phox (NCF1, NOXO2, Neutrophil cytosolic factor 1, 47kD Chronic granulomatous disease, autosomal 1)

Anti -p47 phox (NCF1, NOXO2, Neutrophil cytosolic factor 1, 47kD Chronic granulomatous
disease, autosomal 1)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum B
NADPH Oxidase (nicotinamide adenine dinucleotide phosphate-oxidase) complex is composed of p47 phox (cytosolic component bound to PKC that localizes to p22), p67 phox (cytosolic component that interacts with activated Rac to dock on the glycoprotein p91 subunit (gp91) of membrane bound cytochrome b), Rac2 (Rho family of GTPases), b558 (a cytochrome b transmembrane protein dimer made up of gp91 phox/p22 phox). The activated complex is responsible for the respiratory burst in neutrophils by the multiple phosphorylations of p47 phox by PKC. Neutrophil cytosolic factor 1 (NCF1) is a 47kD cytosolic subunit of the multi-protein complex known as NADPH oxidase. Expression of NCF1 is restricted to granulocytes, monocytes and B lymphocytes. Defects in NCF1 have been reported to be a cause of autosomal recessive chronic granulomatous disease.
Catalog #P1001-24F
ApplicationsSuitable for use in Western Blot and Electron microscopy. Other applications not tested.
Recommended DilutionWestern Blot: 1:500-1:1000. Detects p47-phox in RIPA lysates from HL-60 cells.
Optimal dilutions to be determined by the researcher.
Recommended ControlHL-60 cell lysate
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in buffer, 0.05% sodium azide, 30% glycerol.
PuritySerum
ImmunogenGST fusion protein corresponding to full length human p47-phox
SpecificityRecognizes human p47-phox, Mr ~47kD. Species Crossreactivity: rat. Reported crossreactivity with bovine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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