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Anti -p47 phox (NCF1, NOXO2, p47phox, neutrophil cytosolic factor 1, 47kD, chronic granulomatous disease, autosomal 1, Neutrophil cytosolic factor-1 (47kD)
Anti -p47 phox (NCF1, NOXO2, p47phox, neutrophil cytosolic factor 1, 47kD, chronic
granulomatous disease, autosomal 1, Neutrophil cytosolic factor-1 (47kD)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Serum |
B IP IC |
|
| Neutrophil cytosolic factor 1 (NCF1) is a 47kD cytosolic subunit of the multi-protein complex known as NADPH oxidase. Expression of NCF1 is restricted to granulocytes, monocytes and B lymphocytes. Defects in NCF1 have been reported to be a cause of autosomal recessive chronic granulomatous disease. | | | Catalog # | P1001-24H | | Applications | Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested. | | Recommended Dilution | Western Blot: 1:250-1:1000 detected p47-phox in RIPA lysates from HL-60 cells. | | Immunoprecipitation: 10ul immunoprecipitates p47-phox from 500ug of HL-60 RIPA lysate. | | Immunocytochemistry: 1:250-1:2000 dilution showed positive cytosolic immunostaining for p47-phox in HL-60 cells fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Human | | Concentration | Not determined | | Form | Supplied as a liquid containing 5mM EDTA and 0.02% sodium azide. | | Purity | Serum | | Immunogen | GST fusion protein corresponding to full-length human p47-phox | | Specificity | Recognizes human p47-phox, Mr 47kD. Species Crossreactivity: Reported crossreactivity with bovine. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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