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You are here:Home » Antibodies » Abs to Enzymes, Oxidase » Anti -p67 phox (NCF2, Neutrophil Cytosol Factor 2, 67kD Neutrophil Oxidase Factor, NCF-2, Neutrophil NADPH Oxidase Factor 2, NOXA2)

Anti -p67 phox (NCF2, Neutrophil Cytosol Factor 2, 67kD Neutrophil Oxidase Factor, NCF-2,
Neutrophil NADPH Oxidase Factor 2, NOXA2)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IH
Activation of NADPH-oxidase dependent superoxide generation requires the participation of several cytosolic factors including p47phox, p67phox, p40phox and Rac (1 or 2). In the resting cell, p47phox, p67phox, and p40phox form a large molecular weight complex which can be recovered from the cytosolic fraction. Activation of the NADPH oxidase is initiated by the assembly of p47phox, p67phox, and Rac with cytochrome b558 in a 1:1:1:1 complex in the plasma membrane. p47phox, p67phox, and Rac exhibit cooperative binding, since increasing the concentration of any one component lowers the EC50 of the other components. A great deal of current research involves understanding the protein protein interactions, and how these change with the activation state.
Catalog #P1001-24P
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 0.5-2.0ug/ml detects p67-phox in RIPA lysates from RAW 264.7 cells.
Optimal dilutions to be determined by researcher.
Recommended ControlRat brain tissue, RAW264.7 cell lysate
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceMouse
Concentration~1mg/ml
FormSupplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenSynthetic peptides corresponding to the first 15aa from the N-terminal and the last 15aa on the C-terminal of mouse p67-phox (KLH).
SpecificityRecognizes mouse p67-phox at ~67kD. Species Crossreactivity: rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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