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You are here:Home » Antibodies » Abs to Tumor Suppressors » Anti -p53 (Tumor Protein p53, Tumor Suppressor p53, TP53, TRP53, Cellular Tumor Antigen p53, Phosphoprotein p53, Antigen NY-CO-13, LFS1)

Anti -p53 (Tumor Protein p53, Tumor Suppressor p53, TP53, TRP53, Cellular Tumor Antigen
p53, Phosphoprotein p53, Antigen NY-CO-13, LFS1)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Supernatant B IH FC
p53 protein is to crucial normal regulation of cell growth and is a suppressor of tumor cell proliferation. The gene encoding for p53 is located on chromosome 17p, a fre- quent site of allelic loss in many tumors. A high propor- tion of breast and colon carcinomas are positive for p53 and this expression correlates with poor prognosis in breast cancer. This antibody recognizes both wild type and mutant forms of p53 protein under denaturing and non-denaturing conditions.
Catalog #P1001-27T1
ApplicationsSuitable for use in Flow Cytometry, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:100-1:250
Immunohistochemistry: 1:40 for 1 hr. at RT
Optimal dilutions to be determined by the researcher.
Positive Control
Western Blot: HBL 100 cells
Immunohistochemistry: A high percentage of breast and colonic carcinomas show labeling for p53 protein. In a series of breast carcinomas studied by Cattoretti et al., 45% of the tumors were found to be positive with this antibody (nuclear staining pattern)
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone NoPAb 1801
HostMouse
SourceHuman
ConcentrationNot determined
FormSupplied as a lyophilized powder in 15mM sodium azide. Reconstitute with 1ml dH2O.
PuritySupernatant
ImmunogenHuman p53 beta galactosidase fusion.
SpecificityRecognizes human p53 Protein. Epitope: aa46-55 of the human p53protein.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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