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Specifications

Clone Host Grade Applications Polyclonal Guinea pig Serum B IH IC

Human 62 kD (p62) protein is present in intracytoplasmic inclusions (e.g. hyaline bodies) of hepatocellular carcinoma. p62 protein has been found in many tissues and cells, including lymphoid cells, probably serving a common cellular signal transduction mechanism.
Catalog #	P1001-33Y
Applications	Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended Dilutions	Immunohistochemistry (Frozen and paraffin sections, enhanced after microwave treatment): 1:100-1:200; Incubation Time 1 hr at RT; extended with paraffin sections.
	Western Blot: 1:1000-1:2000 (ECL)
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Guinea pig
Source	Human
Concentration	Not Determined
Form	Supplied as a liquid with 0.09% sodium azide.
Purity	Serum
Immunogen	C-terminal domain, C-NYDIGAALDTIQYSKHPPPL of human p62 protein, conjugated to KLH.
Specificity	Recognizes human p62 protein. Stains neurofibrillary tangles in the brain of patients suffering from Alzheimer’s disease. Species Crossreactivity
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.