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You are here:Home » Antibodies » Abs to Protein Kinases, Ribosomal » Anti -P70 S6 Kinase beta (70kD Ribosomal Protein S6 Kinase 2, EC 2.7.11.1, p70 Ribosomal S6 Kinase beta, p70 S6Kbeta, p70-beta, p70-S6KB, Ribosomal Protein S6 Kinase beta-2, S6 Kinase-related Kinase, S6K-beta, S6K2, Serine/threonine-protein Kinase 14 beta, SRK)

Anti -P70 S6 Kinase beta (70kD Ribosomal Protein S6 Kinase 2, EC 2.7.11.1, p70 Ribosomal
S6 Kinase beta, p70 S6Kbeta, p70-beta, p70-S6KB, Ribosomal Protein S6 Kinase beta-2, S6
Kinase-related Kinase, S6K-beta, S6K2, Serine/threonine-protein Kinase 14 beta, SRK)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified B
P70S6K b is a monomeric, 54kD member of the AGC family of Ser/Thr kinases. It is widely expressed, and found predominately in the nucleus. Although it was initially reported to phosphorylate the ribosomal S6 protein, p70S6Kb/S6Kb2 is now known to also phosphorylate RafB (cell survival), hnRNP (cell proliferation), p300/CBP (transcription) and CDC42 (cytoskeleton stability). Human p70S6Kb/S6Kb2 is 482aa in length. It contains a protein kinase domain (aa67-328), a C-terminal regulatory region (aa329-399) that contains an NLS (aa384-389), and a Pro-rich segment (aa411-482) that likely interacts with SH3 domain-containing proteins. Phosphorylation/dephosphorylation events and sumoylation regulate p70S6Kb activity. In addition to the 54kD form, there is a 56-60kD isoform that possesses a 13aa addition at the N-terminus, plus an isoform that shows a deletion of aa 267-270. Over aa1-410, human p70S6Kb2 shares 95-75aa identity with mouse p70S6Kb2 and human p70S6Kb1, respectively.
Catalog #P1001-38X
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
ConcentrationNot determined
FormSupplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 200ug/ml.
PurityPurified by immunoaffinity chromatography.
ImmunogenRecombinant corresponding to aa2-410 from human P70 S6 Kinase expressed in E. coli (Q9UBS0).
SpecificityRecognizes human P70 S6 Kinase b. Species sequence homology: recombinant human P70 S6 Kinase (3%).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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