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Anti -PACAP-38 (Pituitary Adenylate Cyclase Activating Polypeptide)
Anti -PACAP-38 (Pituitary Adenylate Cyclase Activating Polypeptide)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Serum |
IH IF |
|
| Pituitary adenylate cyclase activating peptide (PACAP), originally isolated from ovine hypothalamus, belongs to the VIP-family of peptides. It occurs in two biologically active forms, PACAP-38 and PACAP-27. PACAP is abundant in the brain, but can be also found in the respiratory and gastrointestinal tracts. Absorption with 10-100ug immunogen per ml diluted antiserum abolishes the staining, while PRP, VIP, PHI, CRF, oxytocin and vasopressin do not. | | | Catalog # | P1775-03 | | Applications | Suitable for use in Immunofluorescence Microscopy and Immunohistochemistry. Other applications not tested. | | Recommended Dilution | Immunofluorescence Microscopy (Frozen sections): 1:400-1:800 with overnight incubation at 4°C. | | Optimal dilutions to be determined by the researcher. | | Positive Control | Stefanini-fixed frozen sections of rat duodenum or hypothalamus. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile 40-50% glycerol, ddH2O. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Concentration | Not determined | | Form | Supplied as a lyophilized powder. Reconstitute in 50-100ul sterile 40-50% glycerol, ddH2O. Dilute further in PBS, 1% BSA, 0.1% sodium azide. | | Purity | Serum | | Immunogen | Synthetic PACAP-38 (BSA) | | Specificity | Recognizes PACAP-38. Some crossreactivity with PACAP-27. Species Crossreactivity: Human, mouse and rat. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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