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Anti -Papilloma Virus, Human, Type 16, Early Protein 7 (hPV)
Anti -Papilloma Virus, Human, Type 16, Early Protein 7 (hPV)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
E B |
|
| Specific types of HPV have been shown to be associated with human cervical cancer. One of these types, HPV16, has been shown to be present in the majority of cervical dysplasias and squamous cell carcinomas studied. Research has also shown that the expression of the HPV16-E7 gene is both sufficient and necessary for cellular transformation in vitro. The immunological detection of HPV types in human samples has been hindered by the lack of an in vitro culture system for the propagation of viruses and viral antigens. Although antibodies that were generated against the bovine papillomavirus capsid antigen (BPV-L1 ORF) are cross-reactive to HPV, these antibodies are of limited use because they do not discriminate among HPV types and have limited sensitivity as compared to standard light microscopic criteria for HPV infection. With bacterial expression systems, fusion proteins of HPV-encoded gene products can be generated and these, in turn, used to develop HPV type specific antibodies. The use of these type specific antibodies to study HPV protein expression may expand understanding of the role of HPV proteins in the pathogenesis of condylomas, dysplasias, and carcinomas of the genital tract. | | | Catalog # | P3105-07D | | Applications | Suitable for use in Western Blot and ELISA. Other applications have not been tested. | | Recommended Dilution | Optimal dilutions to be determined by the researcher. | | Recommended Pair | Suitable for use as a capture antibody. Matched to P3105-07E as a conjugate in sandwich ELISA. | | Hybridoma | SP2/0 myeloma cells with spleen cells of Balb/c mice immunized with HPV oncoprotein E7, type 16. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG2b | | Clone No | 5E733 | | Host | Mouse | | Concentration | ~4mg/ml | | Form | Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide, before the addition of glycerol to 40%. | | Purity | Purified by Protein G affinity chromatography. | | Immunogen | HPV oncoprotein E7, type 16 | | Specificity | Recognizes E7 protein of HPV type 16. Crossreactive with E7 protein of HPV type 18. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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