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You are here:Home » Antibodies » Abs to Infectious Disease Papilloma » Anti -Papilloma Virus, Human, Type 16, Early Protein 7 (hPV)

Anti -Papilloma Virus, Human, Type 16, Early Protein 7 (hPV)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B
Specific types of HPV have been shown to be associated with human cervical cancer. One of these types, HPV16, has been shown to be present in the majority of cervical dysplasias and squamous cell carcinomas studied. Research has also shown that the expression of the HPV16-E7 gene is both sufficient and necessary for cellular transformation in vitro. The immunological detection of HPV types in human samples has been hindered by the lack of an in vitro culture system for the propagation of viruses and viral antigens. Although antibodies that were generated against the bovine papillomavirus capsid antigen (BPV-L1 ORF) are cross-reactive to HPV, these antibodies are of limited use because they do not discriminate among HPV types and have limited sensitivity as compared to standard light microscopic criteria for HPV infection. With bacterial expression systems, fusion proteins of HPV-encoded gene products can be generated and these, in turn, used to develop HPV type specific antibodies. The use of these type specific antibodies to study HPV protein expression may expand understanding of the role of HPV proteins in the pathogenesis of condylomas, dysplasias, and carcinomas of the genital tract.
Catalog #P3105-07E
ApplicationsSuitable for use in Western Blot and ELISA. Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
HybridomaSP2/0 myeloma cells with spleen cells of Balb/c mice
Recommended PairsCapture antibody: P3105-07D
P3105-07E as a conjugate in sandwich ELISA.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No5E734
HostMouse
Concentration~2mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.1% sodium azide, before the addition of glycerol to 40%.
PurityPurified by Protein G affinity chromatography.
ImmunogenHPV oncoprotein E7, type 16
SpecificityRecognizes E7 protein of HPV type 16. Crossreactive with E7 protein of HPV type 18.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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