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You are here:Home » Molecular Biology » MB-Enzymes, Phosphatase » Paraoxonase 1, Recombinant, Human (Paraoxonase-1, PON1, Aromatic Esterase 1, A-esterase 1, Esterase-A, ESA, K-45, Paraoxonase, PON, Serum Aryldialkylphosphatase 1, Serum Paraoxonase/arylesterase 1)

Paraoxonase 1, Recombinant, Human (Paraoxonase-1, PON1, Aromatic Esterase 1, A-esterase 1,
Esterase-A, ESA, K-45, Paraoxonase, PON, Serum Aryldialkylphosphatase 1, Serum
Paraoxonase/arylesterase 1)

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Specifications

Paraoxonase 1 also called Esterase-A is involved in the detoxification of organophosphate insecticides such as parathion. Paraoxonase 1 may also confer protection against coronary artery disease by destroying proinflammatory oxidized lipids present in oxidized low-density lipoproteins (LDLs).
Catalog #P3107-76
Recombinant Human Paraoxonase-1 is expressed in E. coli having a molecular weight of 42.9kD and fused to an amino terminal hexahistidine tag. The biological activity of this product has not yet been tested.
Amino Acid SequenceMAKLIALTLLGMGLALFRNHQSSYQTRLNALREVQPVELPNCNLVKGIETGSEDLEILPNGLAFISSGLKYPGIKSFNPNSPGKILLMDLNEEDPTVLELGITGSKFDVSSFNPHGISTFTDEDNAMYLLVVNHPDAKSTVELFKFQEEEKSLLHLKTIRHKLLPNLNDIVAVGPEHFYGTNDHYFLDPYLRSWEMYLGLAWSYVVYYSPSEVRVVAEGFDFANGINISPDGKYVYIAELLAHKIHVYEKHANWTLTPLKSLDFNTLVDNISVDPETGDLWVGCHPNGMKIFFYDSENPPASEVLRIQNILTEEPKVTQVYAENGTVLQGSTVASVYKGKLLIGTVFHKALYCELZ
ApplicationsSuitable to be used directly as a positive control in Western Blot, ELISA, Immunoprecipitation and other immunological experiments. Other applications not tested.
Recommended Dilutions Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight42.9kD
SourceE. coli
Purity~95% (SDS-PAGE). Single band on Western Blot.
Concentration~0.2mg/ml
FormSupplied as a liquid in PBS, 50% glycerol.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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