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Anti -Parathyroid hormone receptor 1 (PTH/PTHr receptor, PTH1 R)
Anti -Parathyroid hormone receptor 1 (PTH/PTHr receptor, PTH1 R)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Sheep |
Affinity Purified |
E B IH |
|
| PTH1 R (Parathyroid hormone receptor 1; also PTH/PTHr receptor) is a predicted 66-68kD member of the G-protein coupled receptor family 2. It is expressed on a variety of cell types, including hepatocytes, renal epithelium, smooth muscle cells, and osteoblasts plus chondrocytes. PTH1 R is a receptor for both PTH and PTHrP, and PTH binding promotes Ca ++ -release from bone, mediated by osteoclast formation. Mature human PTH1 R is a 7-transmembrane glycoprotein 567 amino acids (aa) in length. It contains a long, ligand-binding N-terminal extracellular region (aa 27-188) and a 130 aa cytoplasmic C-terminal domain. Single aa changes such as Gly121Glu, Ala122Thr and Arg255His can impair PTH1 R signaling. | | | Catalog # | P3109-51C | | Applications | Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. | | Recommended Dilution | ELISA: 0.5-1.0ug/ml | | Western Blot: 1.0ug/ml | | Immunohistochemistry: 3-10ug/ml. Antigen retrieval is recommended. | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Sheep | | Source | Human | | Concentration | ~0.2mg/ml | | Form | Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | CHO cell-derived, recombinant human PTH1 R extracellular domain aa23-189 (Accession #Q03431). | | Specificity | Recognizes human Parathyroid hormone receptor 1. Species sequence homology: mouse, 88%. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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