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You are here:Home » Antibodies » Abs to Hormones, Steroids » Anti -Parathyroid hormone receptor 1 (PTH/PTHr receptor, PTH1 R)

Anti -Parathyroid hormone receptor 1 (PTH/PTHr receptor, PTH1 R)

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Specifications

Clone Host Grade Applications
Polyclonal Sheep Affinity Purified E B IH
PTH1 R (Parathyroid hormone receptor 1; also PTH/PTHr receptor) is a predicted 66-68kD member of the G-protein coupled receptor family 2. It is expressed on a variety of cell types, including hepatocytes, renal epithelium, smooth muscle cells, and osteoblasts plus chondrocytes. PTH1 R is a receptor for both PTH and PTHrP, and PTH binding promotes Ca ++ -release from bone, mediated by osteoclast formation. Mature human PTH1 R is a 7-transmembrane glycoprotein 567 amino acids (aa) in length. It contains a long, ligand-binding N-terminal extracellular region (aa 27-188) and a 130 aa cytoplasmic C-terminal domain. Single aa changes such as Gly121Glu, Ala122Thr and Arg255His can impair PTH1 R signaling.
Catalog #P3109-51C
ApplicationsSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionELISA: 0.5-1.0ug/ml
Western Blot: 1.0ug/ml
Immunohistochemistry: 3-10ug/ml. Antigen retrieval is recommended.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostSheep
SourceHuman
Concentration~0.2mg/ml
FormSupplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
PurityPurified by immunoaffinity chromatography.
ImmunogenCHO cell-derived, recombinant human PTH1 R extracellular domain aa23-189 (Accession #Q03431).
SpecificityRecognizes human Parathyroid hormone receptor 1. Species sequence homology: mouse, 88%.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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