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You are here:Home » Antibodies » Abs to Oncoproteins » Anti -PARK7 (Protein DJ-1, Oncogene DJ1, Parkinson Disease Protein 7)

Anti -PARK7 (Protein DJ-1, Oncogene DJ1, Parkinson Disease Protein 7)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IH
DJ-1 protein has 189aa and was cloned from a HeLa cell cDNA library as a novel oncogene, which weakly transformed NIH3T3 cells. The expression of DJ-1 is induced by growth signals and it is translocated from the cytoplasm to the nucleus during the S phase, even though no nuclear localization signals are present. It has been suggested that DJ-1 is a mitogen-dependent oncogene product involved in Ras-related signal transduction pathway.
Catalog #P3110-99W
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot:
Immunohistochemistry (Formalin fixed paraffin embedded): 1:50 for 30min at RT
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10min followed by cooling at RT for 20min
Optimal dilutions to be determined by the researcher.
Positive ControlNormal Breast
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
HostRabbit
SourceHuman
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.05% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to the C-terminus of human DJ-1.
SpecificityRecognizes human DJ-1. Species Crossreactivity: mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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