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You are here:Home » Antibodies » Abs to Ubiquitin Ligases » Anti -Parkin (RING-type E3 ubiquitin-protein ligase)

Anti -Parkin (RING-type E3 ubiquitin-protein ligase)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Supernatant E B IP IF
Parkin is a ubiquitin-protein isopeptide ligase (E3) involved in ubiquitin/proteasome-mediated protein degradation. Parkin may protect cells from premature death by targeting misfolded or damaged proteins for degradation via the ubiquitin proteasome pathway. Mutations in the parkin gene cause a loss-of-function and/or alter protein levels of parkin. The loss of functional parkin is the most common single cause of autosomal recessive Parkinsonís disease.1-2 Parkin expression is neuron-specific and developmentally regulated, and thus, has a role in neuronal maturation. Parkin may also play roles in cell cycle regulation and tumor suppression.3
Catalog #P3112-03D
ApplicationsSuitable for use in ELISA, Immunoprecipitation, Western Blot and Immunofluorescence. Other applications not tested.
Recommended DilutionELISA: 1:100-1:1000
Immunoprecipitation: 100ul per test
Western Blot: 1:20-1:100
Immunofluorescence: 1:20-1:100
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No6A158
HostMouse
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 0.1% sodium azide.
PurityTissue culture supernatant
ImmunogenFull length recombinant human Parkin
SpecificityReacts with the ~50 kD Parkin protein. Western blots of rat brain may also show a band of unknown origin at ~44 kD. Binds to the second RING domain (C-terminal region) of parkin protein. While the specific 50kD parkin signal was detected in Western blots of high salt fractions of wild-type mouse brain, no signal was detected in parkinnull mouse brain samples. Reactivity has been confirmed with recombinant human Parkin protein, human Parkin-transfected CHO cells, and human, mouse, and rat brain tissue homogenates. Species Sequence Homology: the human Parkin sequence shares ~83% sequence homology with mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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