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You are here:Home » Antibodies » Abs to Ubiquitin Ligases » Anti -Parkin (RING-type E3 ubiquitin-protein ligase)

Anti -Parkin (RING-type E3 ubiquitin-protein ligase)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B
Parkin is a 465 amino acid protein with an N-terminal ubiquitin-like domain, a linker region, a C-terminal TRIAD domain consisting of two RING finger motifs flanking a cysteine-rich IBR (in between RING fingers) motif. Mutations in Parkin are a major cause of autosomal recessive juvenile parkinsonism. Parkin-2 lacks exon 5, which encodes amino acid residues 179-206 of Parkin.
Catalog #P3112-03E
ApplicationsSuitable for use in Western Blot and ELISA. Other applications not tested.
Recommended DilutionWestern Blot: 1-2ug/ml
ELISA: 0.5-1ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No7H90
HostMouse
SourceHuman
ConcentrationAs reported
FormSupplied as a lyophilized powder from a 0.2um filtered solution in PBS, 5% trehalose. Reconstitute with 1ml sterile, ddH2O or sterile 40-50% glycerol.
PurityPurified by Protein G affinity chromatography.
ImmunogenE. coli-derived recombinant human Parkin isoform 2 (aa1-437) Accesssion # NP_604390
SpecificityRecognizes human Parkin, isoforms 1 and 2.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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