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You are here:Home » Antibodies » Abs to Ubiquitin Ligases » Anti -Parkin (RING-type E3 ubiquitin-protein ligase)

Anti -Parkin (RING-type E3 ubiquitin-protein ligase)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IH
Parkin, also known as PARKIN2, is a novel protein in Ubl conjugation pathway. Parkin functions within a multiprotein E3 ubiquitin ligase complex, catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. These substrates include CCNE1, SYT11, GPR37, and STUB1, a 22 kD O-linked glycosylated isoform of SNCAIP and SEPT5. Parkin may play a more general role in the ubiquitin proteasomal pathway by participating in the removal and/or detoxification of abnormally folded or damaged protein. The protein may protect neurons against alpha synuclein toxicity, proteasomal dysfunction, GPR37 accumulation, and kainate-induced excitotoxicity. Defects in PARK2 are a cause of Parkinson disease (PD).
Catalog #P3112-03G
ApplicationsSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionELISA: 0.05-0.2ug/ml
Western Blot: 0.5-1ug/ml
Immunohistochemistry: 5-10ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute by adding sterile 40-50% glycerol, ddH2O. Reconstituted product is stable for 12 months at -20ºC. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a lyophilized powder from PBS, pH 7.4, 0.02% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide within residues 300-350 of human parkin protein (Swiss Prot: O60260). (KLH coupled)
SpecificityRecognizes endogenous levels of human parkin protein. Species Crossreactivity: mouse and rat
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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