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You are here:Home » Antibodies » Abs to Enzymes, Polymerase » Anti -PARP (Poly ADP-Ribose Polymerase)

Anti -PARP (Poly ADP-Ribose Polymerase)

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Supernatant B IP IC IF
PARP, a 116kD nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair predominantly in response to environmental stress (1). This protein can be cleaved by many ICE-like caspases in vitro (2,3) and is one of the main cleavage targets of caspase-3 in vivo (4,5). In human PARP, the cleavage occurs between Asp214 and Gly215, which separates PARP's amino-terminal DNA binding domain (24kD) from its carboxy-terminal catalytic domain (89kD) (2,4). PARP is important for cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis (6).
Catalog #P3113-01N
ApplicationsSuitable for use in Western Blot, Immunoprecipitation and Immunofluorescence/Immunocytochemistry. Other applications not tested.
Recommended DilutionsWestern Blot: 1:1000
Immunoprecipitation: 1:200
Immunofluorescence/Immunocytochemistry: 1:800
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypeMonoclonal
Clone No7H48
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.
PuritySupernatant
ImmunogenSynthetic peptide corresponding to residues surrounding Gly623 of human PARP.
SpecificityRecognizes endogenous levels of total full-length human PARP at ~116kD and the large fragment (89kD) produced by caspase cleavage. Does no react with PARP2 or PARP3. Species Crossreactivity: monkey, mouse, rat
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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