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Specifications

Clone Host Grade Applications Monoclonal Rabbit Supernatant E B IP IF

PARP, a 116kD nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair predominantly in response to environmental stress (1). This protein can be cleaved by many ICE-like caspases in vitro (2,3) and is one of the main cleavage targets of caspase-3 in vivo (4,5). In human PARP, the cleavage occurs between Asp214 and Gly215, which separates PARP's amino-terminal DNA binding domain (24kD) from its carboxy-terminal catalytic domain (89kD) (2,4). PARP is important for cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis (6).
Catalog #	P3113-01N
Applications	Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunofluorescence. Other applications not tested.
Recommended Dilutions	Western Blot: 1:1000
	Immunoprecipitation: 1:100
	Immunofluorescence (IF-IC): 1:400
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Clone No	7H48
Host	Rabbit
Source	Human
Concentration	Not determined
Form	Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.
Purity	Supernatant
Immunogen	Synthetic peptide corresponding to residues surrounding Gly623 of human PARP.
Specificity	Recognizes endogenous levels of total full-length human PARP at ~116kD and the large fragment (89kD) produced by caspase cleavage. Species Crossreactivity: monkey, mouse, rat
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.