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Anti -PARP Cleavage Site, p85, Asp214/Gly215 (Poly ADP-Ribose Polymerase)
Anti -PARP Cleavage Site, p85, Asp214/Gly215 (Poly ADP-Ribose Polymerase)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Rabbit |
Supernatant |
B IP IC FC |
|
| Poly (ADP-ribose) polymerase (PARP) is zinc-dependent DNA binding protein that recognizes DNA strand breaks and is presumed to play a role in DNA repair (1). As a marker for apoptosis, PARP is cleaved in vitro by many caspases, and in vivo by Caspase-3 (2,3). Existing as a 116kD nuclear protein, PARP is cleaved between amino acids Asp214 and Gly215 to yield two fragments of 29kD and 85kD (2,4). | | | Catalog # | P3113-04B | | Applications | Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunocytochemistry. Other applications not tested. | | Recommended Dilution | Flow Cytometry: 1:50 | | Western Blot: 1:1,000 | | Immunocytochemistry: 1:50 | | Immunoprecipitation: 1:50 | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG | | Clone No | 6k307 | | Host | Rabbit | | Source | Human | | Form | Supplied as a liquid. | | Purity | Supernatant | | Immunogen | A synthetic peptide corresponding to residues following the cleavage site of human PARP-1 | | Specificity | Recognizes human PARP-1 | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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