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You are here:Home » Antibodies » Abs to Enzymes, Polymerase » Anti -PARP Cleavage Site, p85, Asp214/Gly215 (Poly ADP-Ribose Polymerase)

Anti -PARP Cleavage Site, p85, Asp214/Gly215 (Poly ADP-Ribose Polymerase)

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Supernatant B IP IC FC
Poly (ADP-ribose) polymerase (PARP) is zinc-dependent DNA binding protein that recognizes DNA strand breaks and is presumed to play a role in DNA repair (1). As a marker for apoptosis, PARP is cleaved in vitro by many caspases, and in vivo by Caspase-3 (2,3). Existing as a 116kD nuclear protein, PARP is cleaved between amino acids Asp214 and Gly215 to yield two fragments of 29kD and 85kD (2,4).
Catalog #P3113-04B
ApplicationsSuitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunocytochemistry. Other applications not tested.
Recommended DilutionFlow Cytometry: 1:50
Western Blot: 1:1,000
Immunocytochemistry: 1:50
Immunoprecipitation: 1:50
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
Clone TypeMonoclonal
IsotypeIgG
Clone No6k307
HostRabbit
SourceHuman
FormSupplied as a liquid.
PuritySupernatant
ImmunogenA synthetic peptide corresponding to residues following the cleavage site of human PARP-1
SpecificityRecognizes human PARP-1
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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