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You are here:Home » Antibodies » Abs to Enzymes, Polymerase » Anti -PARP (Poly ADP-Ribose Polymerase) (BSA & Azide Free)

Anti -PARP (Poly ADP-Ribose Polymerase) (BSA & Azide Free)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IH
Poly ADP-Ribose Polymerase (PARP) uses nicotinamide adenine dinucleotide (oxidized form) NAD as a substrate to catalyse the transfer of ADP-ribose to a variety of nuclear protein acceptors. Proteolysis of PARP to its stable 85kD fragment is an early marker of programmed cell death (apoptosis) and is mediated by the caspase CPP32 protein. Cleavage occurs between Adp216 and Gly217, a site in PARP conserved across species.
Catalog #P3113-06AX
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionsWestern Blot: 5-10ug/ml for 2hrs at RT
Immunohistochemistry (Formalin/paraffin only): 6.25-12.5ug/ml for 30 min at RT. Requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
Optimal dilutions to be determined by the researcher.
Recommended Positive ControlRaji cells, Tonsil
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in 10mM PBS, pH 7.4. Also available with BSA and azide. See P3113-06A.
PurityPurified by Protein A affinity chromatography.
ImmunogenA synthetic peptide for the N-terminal region of human PARP. Molecular Weight
SpecificityRecognizes human poly (ADP-ribose) polymerase in cells undergoing replication or DNA repair. Species Crossreactivity: mouse, rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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