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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified E B

PARP, a 116kD nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair predominantly in response to environmental stress (1). This protein can be cleaved by many ICE-like caspases in vitro (2,3) and is one of the main cleavage targets of caspase-3 in vivo (4,5). In human PARP the cleavage occurs between Asp214 and Gly215, which separates PARP’s N-terminal DNA binding domain (24kD) from its C-terminal catalytic domain (89kD) (2,4). PARP is important for cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis (6).
Catalog #	P3113-07
Applications	Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution	Western Blot: 1:1000
	Immunohistochemistry (Paraffin): 1:50
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Host	Rabbit
Source	Human
Concentration	As reported
Form	Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
Purity	Purified by Protein A and immunoaffinity chromatography.
Immunogen	Synthetic peptide (KLH coupled) corresponding to the caspase cleavage site in PARP.
Specificity	Detects endogenous levels of full length PARP (116kD), as well as the large fragment (89kD) and small fragment (24kD) of PARP resulting from caspase cleavage. Does not crossreact with related proteins. Species Crossreactivity: Human, mouse, rat.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.