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You are here:Home » Antibodies » Abs to Enzymes, Polymerase » Anti -PARP, Cleavage Site (Asp214) (Poly ADP-Ribose Polymerase)

Anti -PARP, Cleavage Site (Asp214) (Poly ADP-Ribose Polymerase)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IC IF
PARP, a 116kD nuclear poly (ADPribose) polymerase, appears to be involved in DNA repair predominantly in response to environmental stress (1). This protein can be cleaved by many ICElike caspases in vitro (2,3) and is one of the main cleavage targets of caspase-3 in vivo (4,5). In human PARP the cleavage occurs between Asp214 and Gly215, which separates PARPís N-terminal DNA binding domain (24kD) from its C-terminal catalytic domain (89kD) (2,4). PARP is important for cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis (6).
Catalog #P3113-07B
Recommended Dilution Western Blot: 1:1000 Immunocytochemistry (ABC) 1:100 Immunocytochemistry (IF) 1:100
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostRabbit
SourceMouse
ConcentrationNot determined
FormSupplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
PurityPurified by Protein A affinity chromatography.
ImmunogenSynthetic peptide (KLH coupled) corresponding to C-terminal residues adjacent to Asp214 in mouse PARP.
SpecificityDetects endogenous levels of the large fragment (89kD) of mouse PARP produced
by caspase cleavage. Does not react with full length PARP.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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