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You are here:Home » Antibodies » Abs to Enzymes, Polymerase » Anti -PARP, Cleaved (Poly ADP-Ribose Polymerase)

Anti -PARP, Cleaved (Poly ADP-Ribose Polymerase)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B FC
The poly (ADP-ribose) polymerase (PARP) is involved in cell recovery from DNA damage, such as methylation of N3-adenine, which activates the base excision repair process. PARP [Poly (ADP-ribose) polymerase] is a 116kD nuclear chromatin-associated enzyme that is cleaved during apoptosis by caspase-3 into a 24kD fragment containing the DNA binding domain and an 89kD fragment containing the catalytic and automodification domains. The 24kD-fragment irreversibly bind to DNA and may contribute to the irreversibility of apoptosis by blocking the access of DNA repair enzymes to DNA strand breaks.
Catalog #P3113-07J
ApplicationsSuitable for use in Western Blot and Flow Cytometry. Other applications not tested.
Recommended DilutionWestern Blot: 1-2ug/ml
Flow Cytometry (intracellular): 1-3ug per test
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG
Clone No4H226
HostMouse
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS containing 0.2% gelatin and 0.05% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenSynthetic peptide containing amino acids near 214/215-cleavage site of human PARP.
SpecificityRecognizes human PARP, Cleaved (Poly ADP-Ribose Polymerase). Specifically detects a 89-kDa cleavage fragment containing the catalytic and auto modification domain.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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