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You are here:Home » Antibodies » Abs to Enzymes, Polymerase » Anti -Poly [ADP-ribose] Polymerase-1 (PARP)

Anti -Poly [ADP-ribose] Polymerase-1 (PARP)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IC
Poly [ADP-ribose] polymerase-1 (PARP) is a nuclear protein that catalyses the poly ADP-ribosylation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. It is activated by DNA strand breaks and plays a role in DNA-base excision repair, DNA-damage signalling, transcription regulation and proteosomal function. PARP is cleaved by caspases during apoptosis to yield two apoptotic fragments, the amino-terminal DNA binding domain (25 kD) and the carboxy-terminal catalytic domain (85 kD). PARP cleavage facilitates the cellular disassembly and serves as a marker of cell undergoing apoptosis.
Catalog #P3113-20Z
ApplicationsSuitable for use in Western Blot and Immunocytochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000
Immunocytochemistry: 1:100
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostRabbit
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in PBS, pH 7.2.
PurityPurified by Protein G affinity chromatography.
ImmunogenSynthetic peptide (GVDEVAKKKSKK) corresponding to aa214-225 of human PARP
SpecificityRecognizes human PARP
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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