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You are here:Home » Antibodies » Abs to Calcium Binding Proteins » Anti -Parvalbumin

Anti -Parvalbumin

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Ascites E B
Calbindins are Ca-binding proteins belonging to the troponin C superfamily. There are two types of CaBPs: the "trigger"- and the "buffer"-CaBPs. The conformation of "trigger" type CaBPs changes upon Ca2+ binding and exposes regions on protein that interact with target molecules, thus altering their activity. The buffer-type CABP are thought to control the intracellular calcium concentration. Parvalbumin (PARV, PVALB or oncomodulin) is a high affinity low mol wt (~12kD) cytoplasmic protein belonging to the troponin C superfamily. It is expressed in high levels only in fast-contracting muscles and at lower levels in brain and several endocrine tissues. PARV has two isoforms: alpha (rat/mouse/human: 110aa, 7p13-p11) and beta form (mouse/rat/human: 109aa) shares ~48% identity (mouse PARVs). The PARV-alpha is less acidic (pI=5) than the beta isoform and has an additional amino acid in the C-terminal helix. PARV-alpha is also expressed in inner ear. PARV-beta isoform is expressed in many fetal tissues, placenta and tumors.
Catalog #P3113-51
ApplicationsSuitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry. Other applications not tested.
Recommended DilutionELISA: 1:10,000-1:100,000. In ELISA, antibody reactivity is Calcium ion dependent.
Western Blot: 1:2000-5000 (ECL). MW: ~12kD.
Immunohistochemistry & Immunofluorescence: 1:500-1:1000; Indirect immunoperoxidase labeling of formalin-fixed, paraffin embedded sections of rat brain.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No3H43
HostMouse
SourceFrog
ConcentrationNot determined
FormSupplied as a lyophilized powder from PBS, 0.05% sodium azide. Reconstitute with 100ul sterile PBS.
PurityAscites
ImmunogenPurified frog muscle Parvalbumin
SpecificityRecognizes frog Parvalbumin. Crossreactivity with respect to the isoforms (PARV-alpha and beta) has not been studied. No significant crossreactivity is seen with other CABPs or EF-hand containing proteins. Species Crossreactivity: bovine, human, mouse, rat, canine, feline and fish.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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