| Calbindins are Ca-binding proteins belonging to the troponin C superfamily. There are two types of CaBPs: the "trigger"- and the "buffer"-CaBPs. The conformation of "trigger" type CaBPs changes upon Ca2+ binding and exposes regions on protein that interact with target molecules, thus altering their activity. The buffer-type CABP are thought to control the intracellular calcium concentration. Parvalbumin (PARV, PVALB or oncomodulin) is a high affinity low mol wt (~12kD) cytoplasmic protein belonging to the troponin C superfamily. It is expressed in high levels only in fast-contracting muscles and at lower levels in brain and several endocrine tissues. PARV has two isoforms: alpha (rat/mouse/human: 110aa, 7p13-p11) and beta form (mouse/rat/human: 109aa) shares ~48% identity (mouse PARVs). The PARV-alpha is less acidic (pI=5) than the beta isoform and has an additional amino acid in the C-terminal helix. PARV-alpha is also expressed in inner ear. PARV-beta isoform is expressed in many fetal tissues, placenta and tumors. |
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| Catalog # | P3113-51 |
| Applications | Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry. Other applications not tested. |
| Recommended Dilution | ELISA: 1:10,000-1:100,000. In ELISA, antibody reactivity is Calcium ion dependent. |
| Western Blot: 1:2000-5000 (ECL). MW: ~12kD. |
| Immunohistochemistry & Immunofluorescence: 1:500-1:1000; Indirect immunoperoxidase labeling of formalin-fixed, paraffin embedded sections of rat brain. |
| Optimal dilutions to be determined by the researcher. |
| Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1 |
| Clone No | 3H43 |
| Host | Mouse |
| Source | Frog |
| Concentration | Not determined |
| Form | Supplied as a lyophilized powder from PBS, 0.05% sodium azide. Reconstitute with 100ul sterile PBS. |
| Purity | Ascites |
| Immunogen | Purified frog muscle Parvalbumin |
| Specificity | Recognizes frog Parvalbumin. Crossreactivity with respect to the isoforms (PARV-alpha and beta) has not been studied. No significant crossreactivity is seen with other CABPs or EF-hand containing proteins. Species Crossreactivity: bovine, human, mouse, rat, canine, feline and fish. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
