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You are here:Home » Antibodies » Abs to Tumor Suppressors » Anti -Patched 1 (PTCH, PTC, BCNS, FLJ42602, Holoprosencephaly 7, HPE7, NBCCS, Patched 1, Patched Homolog 1, Patched Protein Homolog 1, PTC1, PTCH1)

Anti -Patched 1 (PTCH, PTC, BCNS, FLJ42602, Holoprosencephaly 7, HPE7, NBCCS, Patched 1,
Patched Homolog 1, Patched Protein Homolog 1, PTC1, PTCH1)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IH
Patched 1 is a receptor for sonic hedgehog, a member of the hedgehog family of cell signaling proteins. Hedgehog signaling is regulated by Protein patched homolog 1 which keeps the pathway turned off in the absences of activation. This inhibits the function of associated downstream network components such as Smoothened (Smo).
Catalog #P3113-82C4
ApplicationsSuitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended DilutionsWestern Blot: 0.1ug/ml detected Patched 1 on 10ug of human skeletal muscle, fetal tissue lysate.
Immunohistochemistry (Paraffin): 1:300 detected Patched 1 in kidney tissue. Heat induced epitope retrieval with citrate buffer, pH 6.0 is recommended.
Optimal dilutions to be determined by the researcher.
Recommended Secondary AntibodiesI1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb
I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb
I1904-46Q: IgG, H&L (HRP) Pab Gt xRb
Positive ControlHuman skeletal muscle, fetal tissue lysate.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
PurityPurified by affinity chromatography.
ImmunogenLinear peptide corresponding to the cytoplasmic domain of human Protein patched homolog 1, conjugated to KLH.
(Accession #NP_000255)
SpecificityRecognizes human Patched 1 at ~160kD. Species Crossreactivity: mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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