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You are here:Home » Antibodies » Abs to Tumor Suppressors » Anti -Patched 2 (PTCH2, PTC2, Patched Homolog 2, Patched Protein Homolog 2)

Anti -Patched 2 (PTCH2, PTC2, Patched Homolog 2, Patched Protein Homolog 2)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IH
Protein patched homolog 2 is a 180-200kD 12 transmembrane member of the Patched family that exists in a signal cascade with Sonic Hedgehog (SHH) and Smoothened (SMO). Protein patched homolog 2 and Smoothened form a membrane complex that serves as a receptor for Sonic Hedgehog (SHH). Protein patched homolog 2 is 1203 amino acids (aa) in length. It has two terminal cytoplasmic domains, one between aa 1-57, and a second between aa1115-1203.
Catalog #P3113-82J
ApplicationsSuitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended DilutionsWestern Blot: 0.5ug/ml detected Patched 2 on 10ug of HeLa cell lysate.
Immunohistochemistry: 1:1000 detected Patched 2 in human placenta tissue.
Optimal dilutions to be determined by the researcher.
Recommended Secondary AntibodiesI1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb
I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb
I1904-46Q: IgG, H&L (HRP) Pab Gt xRb
Positive ControlHeLa cell lysate
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
PurityPurified by affinity chromatography.
ImmunogenLinear peptide corresponding to human Protein patched homolog 2 at and around the extracellular domain. Conjugated to KLH.
SpecificityRecognizes human Protein patched homolog 2 at ~115-130kD. Species Sequence Homology: mouse, canine and equine, 100%; chicken and bovine, 92%
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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