| Protein patched homolog 2 is a 180-200kD 12 transmembrane member of the Patched family that exists in a signal cascade with Sonic Hedgehog (SHH) and Smoothened (SMO). Protein patched homolog 2 and Smoothened form a membrane complex that serves as a receptor for Sonic Hedgehog (SHH). Protein patched homolog 2 is 1203 amino acids (aa) in length. It has two terminal cytoplasmic domains, one between aa 1-57, and a second between aa1115-1203. |
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| Catalog # | P3113-82J |
| Applications | Suitable for use in Western Blot and Immunohistochemistry. |
| Other applications not tested. |
| Recommended Dilutions | Western Blot: 0.5ug/ml detected Patched 2 on 10ug of HeLa cell lysate. |
| Immunohistochemistry: 1:1000 detected Patched 2 in human placenta tissue. |
| Optimal dilutions to be determined by the researcher. |
| Recommended Secondary Antibodies | I1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb |
| I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb |
| I1904-46Q: IgG, H&L (HRP) Pab Gt xRb |
| Positive Control | HeLa cell lysate |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide. |
| Purity | Purified by affinity chromatography. |
| Immunogen | Linear peptide corresponding to human Protein patched homolog 2 at and around the extracellular domain (KLH). |
| Specificity | Recognizes human Protein patched homolog 2 at ~115-130kD. Species Sequence Homology: mouse, canine and equine, 100%; chicken and bovine, 92% |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
