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Specifications

Clone Host Grade Applications Monoclonal Rabbit Supernatant B IP IH IC

Paxillin is a cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion) (1). Paxillin plays a significant role in integrin signaling and the tyrosine phosphorylation of paxillin is required for integrin-mediated cytoskeletal reorganization (2). Paxillin is phosphorylated by focal adhesion kinase (FAK) at Tyr118 (3). It has been found that phosphorylation of paxillin at both Tyr118 and Tyr31 is essential for MM1 cancer cell migration (4).
Catalog #	P3113-85B
Applications	Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended Dilutions	Western Blot: 1:1000-10,000
	Immunohistochemistry: 1:100
	Immunocytochemistry: 1:250
	Immunoprecipitation: 1:200
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG
Clone No	6k308
Host	Rabbit
Source	Human
Concentration	Not Determined
Form	Supplied as a liquid in 50mM Tris-Glyine, pH 7.4, 0.15M sodium chloride, 0.01% sodium azide, 0.05% BSA, 40% glycerol.
Purity	Supernatant
Immunogen	A synthetic peptide corresponding to residues in the N-terminus of human Paxillin.
Specificity	Recognizes alpha, beta and gamma isoforms of human Paxillin at ~65kD. Species Crossreactivity: mouse and rat.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.