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You are here:Home » Antibodies » Abs to Paxillin » Anti -Paxillin (BSA & Azide Free) (PXN)

Anti -Paxillin (BSA & Azide Free) (PXN)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP IH IF
Paxillin is a focal adhesion protein and a substrate for several tyrosine kinases such as src, FAK, and p210BRC/ABL. The tyrosine phosphorylation of paxillin is affected by conditions that change cell-cell adhesion. Paxillin associates tightly with FAK and Crk through its SH2 domain. This interaction is independent of the extracellular matrix. Although paxillin was initially discovered in fibroblasts, its phosphorylation may also be important during neurite extension during differentiation.
Catalog #P3113-86X
MW of Antigen 68kD
Epitope Not determined
Applications and Recommended DilutionsImmunofluorescence
Immunoprecipitation (Native verified)
(Use Protein G) (Ab 2ug/mg protein lysate)
Western Blotting (Ab 1-2ug/ml for 2hrs at RT))
Immunohistochemistry (Formalin/paraffin) (Ab 1-2ug/ml for 30 min at RT) * [Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 8.0, for 10-20 min followed by cooling at RT for 20 min.] The optimal dilution for a specific application should be determined by the investigator.
Positive Control HeLa cells. Colon carcinoma.
Cellular Localization Cell membrane and cytoplasmic
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at-20°C or colder. Aliquots are stable for at least 12 months at-20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No3F312
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in 10mM PBS, pH 7.4. Also available with BSA and azide. See P3113-86
PurityPurified by Protein G affinity chromatography.
ImmunogenPuri ed recombinant full-length human paxillin protein
SpecificityRecognizes Paxillin. Species Crossreactivity: human and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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