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You are here:Home » Antibodies » Abs to Paxillin » Anti -Paxillin (PXN)

Anti -Paxillin (PXN)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B
Paxillin, a focal adhesion protein, is involved in focal adhesion formation during cell adhesion and migration. Paxillin contains LD motifs, LIM domains, and SH3-/SH2-binding domains that participate in a variety of proteinprotein interactions with kinases, GTPase-activating
proteins, and cytoskeletal proteins. Phosphorylation of paxillin occurs at both tyrosine and serine sites. Serine phosphorylation of paxillin occurs in response to growthfactor activation and fibronectins. Both JNK1 and cdc2kinases can phosphorylate serine 178 in paxillin. The mutant form of paxillin (S178A) decreases the migration of keratocytes and epithelial cells. Thus, phosphorylation paxillin at serine 178 may be important during cell migration.
Catalog #P3113-87
ApplicationsSuitable for use in ELISA, Western Blot or for antigen applications in immunological protocols. Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No5i46
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenFull-length recombinant human paxillin.
SpecificityDetects a 68kD protein corresponding to the molecular mass of paxillin on SDS-PAGE
immunoblots of A431 cells. Similar results were seen in various cells and tissues from rat, mouse, and chicken origin.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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