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You are here:Home » Antibodies » Abs to Paxillin » Anti -Paxillin, phosphorylated, Ser178 (PXN)

Anti -Paxillin, phosphorylated, Ser178 (PXN)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
Paxillin, a focal adhesion protein, is involved in focal adhesion formation during cell adhesion and migration. Paxillin contains LD motifs, LIM domains, and SH3-/SH2-binding domains that participate in a variety of proteinprotein interactions with kinases, GTPase-activating proteins, and cytoskeletal proteins. Phosphorylation of paxillin occurs at both tyrosine and serine sites. Serine phosphorylation of paxillin occurs in response to growthfactor activation and fibronectins. Both JNK1 and cdc2 kinases can phosphorylate serine 178 in paxillin. The mutant form of paxillin (S178A) decreases the migration of keratocytes and epithelial cells. Thus, phosphorylation paxillin at serine 178 may be important during cell migration.
Catalog #P3113-87A
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:500
ELISA: 1:2000
Optimal dilutions to be determined by the researcher.
Storage and StabilityFor long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenPhospho-Paxillin (Ser-178) synthetic peptide (coupled to carrier protein) corresponding to amino acid residues around serine 178 of human paxillin. This human sequence is highly conserved in rat and mouse paxillin.
SpecificityDetects a 68kDa protein corresponding to the molecular mass of phosphorylated paxillin on SDSPAGE immunoblots of EGF treated A431 cells, but not in A431 control cells. Similar results were seen in calyculin A treated human A431 and aortic endothelial cells.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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