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You are here:Home » Antibodies » Abs to Paxillin » Anti -Paxillin, phosphorylated (Ser17)

Anti -Paxillin, phosphorylated (Ser17)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
Paxillin, a focal adhesion protein, is involved in focal adhesion formation during cell adhesion and migration. Paxillin contains LD motifs, LIM domains, and SH3-/SH2-binding
domains that participate in a variety of protein-protein interactions with kinases, GTPase-
activating proteins, and cytoskeletal proteins. Phosphorylation of paxillin occurs at both
tyrosine and serine sites. Serine phosphorylation of paxillin occurs in response to growth-
factor activation and fibronectins. Both JNK1 and cdc2 kinases can phosphorylate serine
178 in paxillin. The mutant form of paxillin (S178A) decreases the migration of keratocytes
and epithelial cells. Thus, phosphorylation paxillin at serine 178 may be important during
cell migration.
Catalog #P3113-87C
ApplicationsSuitable for use in Western Blot and ELISA. Other applications not tested.
Recommended DilutionWestern Blot: 1:500
ELISA: 1:2000
Optimal dilution to be determined by researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostRabbit
FormSupplied as a liquid in PBS, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide.
PurityPurified by immunoaffinity chromatography
ImmunogenSynthetic peptide (coupled to KLH) corresponding to amino acid residues around serine 178 of human paxillin. This human sequence is highly conserved in rat and mouse paxillin.
SpecificityThis antibody detects a 68kD protein corresponding to the molecular weight of phosphorylated paxillin on SDS-PAGE immunoblots of EGF treated A431 cells, but not in
A431 control cells. Similar results were seen in calyculin A treated human A431 and aortic
endothelial cells. EpitopePhosphoserine 178
Species Cross ReactivityHuman
This human sequence is highly conserved in rat and mouse paxillin.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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