| Paxillin, a focal adhesion protein, is involved in focal adhesion formation during cell adhesion and migration. Paxillin contains LD motifs, LIM domains, and SH3-/SH2-binding |
|
| domains that participate in a variety of protein-protein interactions with kinases, GTPase- |
|
| activating proteins, and cytoskeletal proteins. Phosphorylation of paxillin occurs at both |
|
| tyrosine and serine sites. Serine phosphorylation of paxillin occurs in response to growth- |
|
| factor activation and fibronectins. Both JNK1 and cdc2 kinases can phosphorylate serine |
|
| 178 in paxillin. The mutant form of paxillin (S178A) decreases the migration of keratocytes |
|
| and epithelial cells. Thus, phosphorylation paxillin at serine 178 may be important during |
|
| cell migration. |
|
| Catalog # | P3113-87C |
| Applications | Suitable for use in Western Blot and ELISA. Other applications not tested. |
| Recommended Dilution | Western Blot: 1:500 |
| ELISA: 1:2000 |
| Optimal dilution to be determined by researcher. |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Host | Rabbit |
| Form | Supplied as a liquid in PBS, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. |
| Purity | Purified by immunoaffinity chromatography |
| Immunogen | Synthetic peptide (coupled to KLH) corresponding to amino acid residues around serine 178 of human paxillin. This human sequence is highly conserved in rat and mouse paxillin. |
| |
| Specificity | This antibody detects a 68kD protein corresponding to the molecular weight of phosphorylated paxillin on SDS-PAGE immunoblots of EGF treated A431 cells, but not in |
| A431 control cells. Similar results were seen in calyculin A treated human A431 and aortic |
| endothelial cells. Epitope | Phosphoserine 178 |
| Species Cross Reactivity | Human |
| This human sequence is highly conserved in rat and mouse paxillin. |
|
|
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
