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Specifications

Clone Host Grade Applications Monoclonal Rabbit Supernatant B IP IH IC

Paxillin is a cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion) (1). Paxillin plays a significant role in integrin signaling and the tyrosine phosphorylation of paxillin is required for integrin-mediated cytoskeletal reorganization (2). Paxillin is phosphorylated by focal adhesion kinase (FAK) at Tyr118 (3). It has been found that phosphorylation of paxillin at both Tyr118 and Tyr31 is essential for MM1 cancer cell migration (4).
Catalog #	P3113-87D
Applications	Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry. Other applications not tested.
Recommended Dilution	Western Blot: 1:10,000
	Immunohistochemistry: 1:100-1:250
	Immunocytochemistry: 1:250
	Immunoprecipitation: 1:100
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG
Clone No	6k309
Host	Rabbit
Source	Human
Form	Supplied as a liquid.
Purity	Supernatant
Immunogen	A synthetic phospho-peptide corresponding to residues surrounding Tyr31 of human Paxillin
Specificity	Recognizes human Paxillin when phosphorylated at Tyr31.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.