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You are here:Home » Antibodies » Abs to Paxillin » Anti -Paxillin (PXN)

Anti -Paxillin (PXN)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified E B IP IH IC
Paxillin can be diffusely distributed in the cytoplasm, co-localized with actin at the cell periphery, or aggregated at focal adhesions. Paxillin has several motifs which allow its interaction with other proteins. These motifs include LD motifs, LIM domains, an SH3 domain binding site, and SH2 domain binding sites. Paxillin complexes with proteins of focal adhesions including vinculin, talin, with the kinases FAK, PYK2, Src, and Csk, with ARF GTPase activating proteins, and with the adaptor protein v-Crk.
Catalog #P3113-90A
Paxillin is phosphorylated at several residues, including tyrosine 31, tyrosine 118, tyrosine 181. The phosphorylation of paxillin appears to be important for several biological functions including S-phase transition and neurite extension.
ApplicationsWestern blot: 1:10001:10,000
ELISA
Immunohistochemistry
Immunocytochemistry
Immunoprecipitation
Control Lysate A431 Lysate: Prepared from a human epidermoid carcinoma cell line. Cell lysate is available to be used as a positive control in Western blotting. Cell lysate is provided at a concentration of 1ug/ml in SDS-PAGE buffer (62mM Tris pH 6.8, 2% SDS, 0.9% b- mercaptoethanol, 0.003% bromphenol blue and 5% glycerol). 510ul/lane is sufficient for Western blot
Clone TypeMonoclonal
IsotypeIgG1
Clone No2Q1106
HostMouse
SourceChicken
Concentration~1mg/ml
FormIn 50% glycerol, 20mM sodium phosphate, pH 7.5, 150mM sodium chloride, 1.5mM sodium azide.
PurityPurified IgG
ImmunogenAffinity purified phosphotyrosine-containing proteins from Rous sarcoma virustransformed chick embryo fibroblasts
SpecificityReacts strongly with two bands on Western blots of total cell lysate from chick embryo fibroblasts or rat brain. The lower molecular band is believed to be either an isoform or a degradation product of the 68kD protein, since it is found also on SDS-PAGE of affinitypurified paxillin. Recognizes human, canine, mouse, chick and rat.
Important NoteThis product is intended for research use only, not for use in human, therapeutic or diagnostic applications.


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