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You are here:Home » Antibodies » Abs to Paxillin » Anti -Paxillin (PXN)

Anti -Paxillin (PXN)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP IH IF
Paxillin can be diffusely distributed in the cytoplasm, co-localized with actin at the cell periphery, or aggregated at focal adhesions. Paxillin has several motifs which allow its interaction with other proteins. These motifs include LD motifs, LIM domains, an SH3 domain binding site, and SH2 domain binding sites. Paxillin complexes with proteins of focal adhesions including vinculin, talin, with the kinases FAK, PYK2, Src, and Csk, with ARF GTPase activating proteins, and with the adaptor protein v-Crk.
Catalog #P3113-90B
Paxillin is phosphorylated at several residues, including tyrosine 31, tyrosine 118, tyrosine 181. The phosphorylation of paxillin appears to be important for several biological functions including S-phase transition and neurite extension.
Hybridoma Produced by the fusion between BALB/c mouse splenocytes and mouse myeloma NS1 cells.
ApplicationsSuitable for use in Immunofluorescence, Western Blotting, Immunoprecipitation, Immunohistochemistry. Other applications not tested.
Recommended DilutionImmunofluorescence:
Western Blotting: 1.0ug/ml with incubation for 2 hours at RT
Immunoprecipitation: 2.0 ug/mg of protein lysate
Immunohistochemistry: 1.0-2.0ug/ml, incubation for 30 minutes at RT (on acetone-fixed frozen and formalin-fixed/paraffin-embedded tissue sections)
Staining of formalin/paraffin tissues requires boiling tissue sections in 1 mM EDTA, pH 8.0, for 10-20 minutes followed by cooling at RT for 20 minutes.
Optimal dilutions to be determined by the researcher.
Recommended Positive Control A431 or HeLa cells. Tonsil tissue. Breast or colon carcinomas.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No2B2.75
HostMouse
SourceHuman
Concentration~0.2mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.2% BSA, 15mM sodium azide
PurityPurified from ascites by Protein G affinity chromatography.
ImmunogenPurified recombinant full-length human paxillin protein.
SpecificityRecognizes paxillin, a protein with Mr68kD. Species Reactivity: Human and rat.
Important NoteThis product is intended for research use only, not for use in human, therapeutic or diagnostic applications.


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