Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Antibodies » Abs to Paxillin » Anti -Paxillin (PXN)

Anti -Paxillin (PXN)

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
Paxillin is a multi-domain protein that localizes primarily in focal adhesion sites to the extracellular matrix (1). Paxillin is one of the key components of integrin signaling and tyrosine phosphorylation of paxillin is required for integrin-mediated cytoskeletal reorganization (2). Paxillin is phosphorylated by another focal adhesion component, focal adhesion kinase (FAK), at tyrosine 118 (3,4). Phospho-Tyr118 may provide a docking site for recruitment of other signaling molecules to focal adhesions. It has been shown that the
SH2 domain of Crk binds to the phosphorylated Tyr118 of paxillin (5).
Catalog #P3113-90H
ApplicationsSuitable for use in Western Blotting. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (30-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol
PurityProtein A and peptide affinity chromatography
ImmunogenSynthetic peptide (KLH coupled) corresponding to residues near the carboxy- terminal sequence of human paxillin
SpecificityDetects endogenous levels of paxillin from various cell lines. Does not crossreact with other related proteins
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links