| Paxillin is a 68kD cytoskeletal adapter protein involved in organization and function of focal |
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| adhesions, which are critical to cell adhesion and migration. This in turn plays a role in a wide |
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| variety of processes including embryogenesis, organogenesis, wound repair, inflammation and |
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| cancer. Paxillin contains LD motifs, LIM domains, SH3 and SH2 binding domains that serve as |
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| docking sites for cytoskeletal proteins, tyrosine kinases (e.g., FAK, Pyk2, Src), serine/threonine |
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| kinases, GTPase activating proteins and other adaptor proteins (e.g., actin, vinculin, Crk). Tyrosine |
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| 31, a Crk binding site of paxillin, is phosphorylated during integrin-mediated cell adhesion. |
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| Catalog # | P3113-90Y |
| Applications | Suitable for use in ELISA and Western Blot. Other applications not tested. |
| Recommended Dilution | Western Blot: 1:1000 |
| Optimal dilutions to be determined by the researcher. |
| Positive Control | NMuMG cells |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Host | Rabbit |
| Source | Human |
| Concentration | Not determined |
| Form | Supplied as a liquid in PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free), pH 7.2, 0.05% sodium azide, 50% glycerol. |
| Purity | Purified by immunoaffinity chromatography. |
| Immunogen | Synthetic peptide corresponding to human paxillin that contains tyrosine 31. |
| Specificity | Recognizes mouse paxillin when phosphorylated at Tyr31. Species sequence homology: Human (100%) and chicken (93%). |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
