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You are here:Home » Antibodies » Abs to Paxillin » Anti -Paxillin, phosphorylated, Tyr118 (PXN)

Anti -Paxillin, phosphorylated, Tyr118 (PXN)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IF
Paxillin is a multi-domain protein that localizes primarily to focal adhesion sites to the extracellular matrix (1). Paxillin is one of the key components of integrin signaling and tyrosine phosphorylation of paxillin is required for integrin-mediated cytoskeletal reorganization (2). Paxillin is phosphorylated by another focal adhesion component, focal adhesion kinase (FAK), at tyrosine 118 (3,4). Phospho-Tyr118 may provide a docking site for recruitment of other signaling molecules to focal adhesions. It has been shown that the SH2 domain of Crk binds to the phosphorylated Tyr118 of paxillin (5).
Catalog #P3113-91
ApplicationsSuitable for use in ELISA, Western Blot and Immunofluorescence. Other applications have not been tested.
Recommended DilutionWestern Blot: 1:1000.
Immunofluorescence (IC): 1:50
Optimal dilutions to be determined by researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
PurityPurified by Protein A affinity chromatography.
ImmunogenSynthetic phosphopeptide corresponding to residues surrounding Tyr118 of human paxillin (KLH).
SpecificityRecognizes endogenous human paxillin only when phosphorylated at Tyr118 at ~68kD. Does not crossreact with other tyrosine phosphorylated proteins. Species Crossreactivity: mouse, rat and monkey.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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