Anti -Paxillin, phosphorylated (Ser126) (Paxillin alpha, PXN, PXN Protein, FLJ16691,
FLJ23042)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
B |
|
| Paxillin is a 68kD cytoskeletal adapter protein involved in organization and function of focal adhesions, which are critical to cell adhesion and migration. These cellular events in turn play a role in a wide variety of processes including embryogenesis, organogenesis, wound repair, inflammation and cancer. Paxillin contains LD motifs, LIM domains, SH3 and SH2 binding domains that serve as docking sites for cytoskeletal proteins, serine kinases (e.g., FAK, Pyk2, Src), serine/threonine kinases, GTPase activating proteins and other adaptor proteins (e.g., actin, vinculin, Crk). Serine 126 of paxillin is phosphorylated as a result of Raf stimulation, through the Raf --> MEK --> ERK pathway. | | | Catalog # | P3113-91D | | Positive Control | RAW 264.7 cells treated with LPS; 293 cells transfected with wild-type EGFP-tagged human paxillin. | | Applications | Suitable for use in Western Blot. Other applications not tested. | | Recommended Dilution | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Host | Rabbit | | Source | Human | | Concentration | As reported | | Form | As reported | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | Synthetic phosphopeptide derived from the region of human paxillin that contains serine 126. | | Specificity | Recognizes human Paxillin when phosphorylated at Ser126. Species Crossreactivity: Mouse (100% homologous) paxillin. Chicken (100% homologous) has not been tested, but is expected to react. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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