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You are here:Home » Antibodies » Abs to Paxillin » Anti -Paxillin, phosphorylated (Ser178) (PXN)

Anti -Paxillin, phosphorylated (Ser178) (PXN)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
Paxillin, a focal adhesion protein, is involved in focal adhesion formation during cell adhesion and migration. Paxillin contains LD motifs, LIM domains, and SH3-/SH2-binding domains that participate in a variety of protein-protein interactions with kinases, GTPase-activating proteins, and cytoskeletal proteins. Phosphorylation of paxillin occurs at both tyrosine and serine sites. Serine phosphorylation of paxillin occurs in response to growth-factor activation and fibronectins. Both JNK1 and cdc2 kinases can phosphorylate serine 178 in paxillin. The mutant form of paxillin (S178A) decreases the migration of keratocytes and epithelial cells. Thus, phosphorylation paxillin at serine 178 may be important during cell migration.
Catalog #P3113-91U
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:500
Optimal dilutions to be determined by the researcher.
Positive ControlEGF treated A431 cell lysate, calyculin A treated human A431, or aortic endothelial cells.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in PBS, 1mg/ml BSA, 0.05% sodium azide, 50% gylcerol.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to aa residues around serine 178 of human paxillin (KLH).
SpecificityRecognizes human Paxillin when phosphorylated at Ser178. Species Crossreactivity: mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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