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You are here:Home » Antibodies » Abs to Paxillin » Anti -Pax2 (Paired Box Gene 2, Paired Box Homeotic Gene 2, Paired Box Protein 2, Paired Box Protein Pax-2, Paired Box Protein Pax2, Pax-2)

Anti -Pax2 (Paired Box Gene 2, Paired Box Homeotic Gene 2, Paired Box Protein 2, Paired
Box Protein Pax-2, Paired Box Protein Pax2, Pax-2)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IP IH
The pax genes are a family of transcription factors that are active in specific tissues during early embryonic development. Pax family members possess a DNA-binding domain encoded by the paired box. Because paired domain containing genes encode transcription factors, they are capable of executing a genetic program. The paired box containing genes were first identified in Drosophila and were subsequently described in a variety of species including mouse, human, chicken, Xenopus and Zebrafish. Several pax genes have also been associated with developmental mutations including: pax-3, which is associated with Waardenburg syndrome, pax-6 is associated with Aniridia, and pax-2 which is associated with Wilms tumor. During embryogenesis, Pax-2 is expressed in the developing kidney. In particular, the pax-2 gene is expressed in condensing metanephric mesenchyme and in early epithelial structures derived from mesenchyme; however, pax-2 mRNA and protein levels are rapidly down regulated as the tubular epithelium matures. Although Pax-2 is down regulated during renal epithelium maturation, Pax-2 expression persists in the undifferentiated epithelium of Wilms’ tumors. Persistent expression of Pax-2 in Wilms’ tumors occurs frequently and correlates with the proliferation of poorly differentiated epithelial cells in these tumors. Interestingly, expression of the Wilms’ tumor suppresser protein WT1 coincides with down-regulation of the pax-2 gene, and the WT1 protein can directly repress pax-2 transcription. Therefore, pax-2 is thought to be a target gene for WT1.
Catalog #P3113-97
ApplicationsSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionsELISA: 0.1-1ug/ml
Western Blot: 1ug/ml
Immunohistochemistry: 5ug/ml (whole mount staining)
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceMouse
Concentration~0.25mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenA GST-Pax-2 fusion protein corresponding to aa188-385 from the C-terminal of mouse Pax2.
SpecificityRecognizes mouse Pax2. Does not crossreact with other Pax family members. The major epitopes are located between aa204-373 of mouse Pax2. Two proteins are detected in mouse embryonic kidney lysates
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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