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You are here:Home » Antibodies » Abs to Obesity Proteins » Anti -PBEF (Pre-B cell Colony Enhancing Factor, Pre-B cell Enhancing Factor, 1110035O14Rik, DKFZP666B131, EC 2.4.2.12, MGC117256, Nicotinamide Phosphoribosyltransferase, NAmPRTase, NAMPT, Pre-B cell Colony Enhancing Factor 1, PBEF1, Visfatin)

Anti -PBEF (Pre-B cell Colony Enhancing Factor, Pre-B cell Enhancing Factor,
1110035O14Rik, DKFZP666B131, EC 2.4.2.12, MGC117256, Nicotinamide
Phosphoribosyltransferase, NAmPRTase, NAMPT, Pre-B cell Colony Enhancing Factor 1, PBEF1,
Visfatin)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified E B IP IH IC
Visfatin is pre-B cell colony-enhancing factor 1 (PBEF), which is predominantly secreted from visceral adipose tissue both in humans and mice. This protein has also been reported to be a cytokine (PBEF) that promotes B cell maturation and inhibits neutrophil apoptosis, or a visceral-fat derived hormone that acts by binding and activating the insulin receptor.
Catalog #P3113-97J
ApplicationsSuitable for use in ELISA, Western Blot, Immunohistochemistry, Immunoprecipitation and Immunocytochemistry. Other applications not tested.
Recommended DilutionsELISA (Direct and indirect): 1:2000-1:5000
Western Blot: 1:2000-1:5000 using ECL; suggested blocking and dilution buffer is PBST containing 0.05% Tween-20 and 5% skim milk; suggested incubation time is 1 hour at RT.
Immunohistochemistry (Paraffin): 1:500-1:1000
Immunoprecipitation: 1:500-1:1000
Immunocytochemistry: 1:500-1:1000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No6D71
HostMouse
SourceHuman
Concentration~1mg/ml
FormSuppied as a liquid in PBS, pH 7.4. Preservative-free.
PurityPurified
ImmunogenRecombinant human Visfatin.
SpecificityRecognizes human Visfatin at ~54kD by Western Blot. Species Crossreactivity: rat and mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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